Ingredient Repository · Ingredient

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Ingredient

Protease

A broad-spectrum proteolytic enzyme that hydrolyzes dietary proteins into absorbable amino acids and peptides.

What it is

Protease (peptidase or proteinase) refers to a group of enzymes that cleave peptide bonds linking amino acids in protein molecules. Supplemental proteases are typically produced via fungal fermentation (Aspergillus niger or Aspergillus oryzae) and include acid, neutral, and alkaline proteases. This multi-pH stability allows supplemental protease to function in acidic gastric juices as well as the neutral-to-alkaline duodenum, initiating protein breakdown earlier than pancreatic trypsin.

Also known as: Acid Protease, Digestive Protease

What it does

  • Hydrolyzes complex dietary proteins into bioactive peptides and free amino acids.
  • Reduces postprandial fullness, gas, and putrefactive fermentation caused by undigested protein.
  • Enhances overall protein bioaccessibility and systemic amino acid bioavailability.

Amplified by probiotics

Complete protein digestion by exogenous proteases minimizes the transit of large, undigested dietary protein blocks into the lower intestine, preventing putrefactive bacterial fermentation that produces toxic amines like ammonia and indole. Protease releases essential amino acids that serve as structural building blocks for live probiotic bacteria. In return, probiotics ferment residual peptides into beneficial short-chain fatty acids (SCFAs), lower gut pH, and upregulate mucin synthesis (MUC2). This protective mucus layer shields the enterocyte surface, promotes tissue healing, and supports efficient active transport systems for digested amino acid uptake.

Typical dose

10,000–50,000 HUT/day

The evidence

Evidence: strong

Randomized human trials demonstrate that oral fungal protease supplementation significantly increases postprandial plasma amino acid concentrations, speeds gastric emptying, and reduces functional indigestion symptoms (Oben et al. 2008, J Int Soc Sports Nutr; Roxas 2008, Altern Med Rev).

  1. Oben J, et al. An open label study to determine the effects of an oral proteolytic enzyme blend on whey protein digestion in healthy subjects. J Int Soc Sports Nutr. 2008;5:10. PubMed ↗
  2. Roxas M. The role of enzyme supplementation in digestive disorders. Altern Med Rev. 2008;13(4):307-314. PubMed ↗

Safety & who should check first

Exceedingly high safety profile; well-tolerated across broad dosage ranges. Rare allergic responses in workers handling raw enzyme dust; oral capsules are benign.