Skip to content

Heat stability and shelf life

Effect of Microencapsulation on Probiotics Heat Stability and Shelf-life

Lab.

Introduction

This research was carried out on Microencapsulation with scCO 2 technology to demonstrate the extended shelf life provided by Velobiotics™ (Microencapsulated Probiotics).

The  probiotic industry faces the challenge of  retention of probiotic culture viability as numbers of these cells within their products inevitably decrease over time. In order to retain probiotic viability levels above the therapeutic minimum over the duration of the product’s shelf life, various methods have been employed, among which encapsulation has received much interest.

The microparticles produced through  Velobiotics™ encapsulation protected the bacteria in simulated gastrointestinal fluids.

This study reports on accelerated shelf life studies of PVP:PVAc-CA encapsulated Bifidobacterium lactis Bb12 and Bifidobacterium longum Bb46. Samples were stored as free powders in glass vials at 30 _C for 12 weeks and then analyzed for viable counts and water activity levels weekly or fortnightly.

Water activities of the samples were within the range of 0.25–0.43, with an average aw = 0.34, throughout the storage period. PVP:PVAc-CA interpolymer complex encapsulation retained viable levels above the recommended minimum for 10 and 12 weeks, for B. longum Bb46 and B. lactis Bb12, respectively, thereby extending their shelf lives under high storage temperature by between 4 and 7 weeks.

One of the primary challenges with most Probiotics is the degradation of live bacteria at room temperatures during storage in the pharmacy. This results in  lower than expected load of CFU (Colony Forming Units) that is meant to confer a health benefit.

The viability of the microorganism is defined as the number of viable and active cells per gram or ml of probiotic food or supplement at the moment of consumption. The importance of this number is because it determines the medicinal efficacy of the probiotic bacteria.

Therefore in order to maintain high consumer confidence in probiotic bacteria being consumed, it is important to ensure high survival rate of bacteria during production as wells as over the product shelf life. 

Improving the viability of probiotic bacteria in different food products until the time of consumption has been the subject of many studies and innovations.

Supercritical CO2 interpolymer complex encapsulation improves heat stability of probiotic bifidobacteria

M. S. Thantsha • P. W. Labuschagne •C. I. Mamvura

Conclusions

  • Microencapsulation appreciably improved viability of bifidobacteria at 30 degrees Celcius over the test period.
  • Velobiotics™ therefore allows the  supply of a stable probiotic formulation in areas where proper storage, refrigeration or degradation at room temperature is common.

Full Study

Based on 885 reviews
86%
(762)
8%
(70)
2%
(21)
1%
(11)
2%
(22)
20s
It makes me confident during my

It makes me confident during my workouts

Very Happy so far with the GP1

Very Happy so far with the GP1. I ordered the tummy toner and the collagen
and the digestive capsules will received yesteday started today will let you know.

N
Femina COMBO Care
Natasha Elenie Ferreira
Im totally satisfied

Im totally satisfied. Love the products

The best product ever👏🏼😃

I absolutely love this product, it’s working wonders on me🥹👏🏼😃I’m literally recommending it to everyone I know.

Great results once again

Great results once again 🙏thanks

30 Chewable Tablets
They have helped me alot

They have helped me a lot

I'm the girl

I've been experiencing smelly discharge after my periods and during, but since I have started using this products,I have regained my confidence. Thanks Velobiotics

30 Chewable Tablets

The best!

Too soon to tell for skin and hair but my gut is

Too soon to tell for skin and hair but my gut is very happy

30 Chewable Tablets
We love them

We love them ❤️

F
BioKid™ Probiotics Chewables
Fanelesibonge Makhathini
30 Chewable Tablets
Super awesome

Am so happy using biokid it’s absolutely the best probiotics my son is no longer feeling bloated and having tummy aches. All thanks to Biokid 😍

i love the capsules

i love the capsules, my stomach feels less gassy since i started taking them

The girl she is.

The infection started coming out the next day.
I highly recommend.
I'm so glad that I bought this it'll be my go to from now on.
Ladies go grab yourself one.

30 Capsules
I’m seeing & feeling the difference

I’m seeing & feeling the difference. Lepousse is healthier & yeast infections are kept at bay.

Do not purchase

No positive effects at all. A complete waste of money.

I'm seeing really really good results with the Gut Slim Probiotic SuperGreens

I'm seeing really really good results with the Gut Slim Probiotic SuperGreens.. It's been 4 weeks now. Very impressed. Sadly delivery took over a month. I'll probably order again.

Side note... Based on my research this product is the South African equivalent of the famous neurobiologist Dr Andrew Huberman's daily drink AG1 Greens. Again, seeing good results.

20s
Great product

Great product, it works wonders.

What an amazing product

What an amazing product, probiotics were definitely the missing piece in my wellness journey.🌸💫

Been good for me for 2 weeks now

Been good for me for 2 weeks now.

Y
GutSlim Probiotics Diet Shake
Yvonette Theunisen
Great shake

Great shake ,doesn’t have a bad taste and it helps me control my meals as someone who use to struggle with eating,it helps me eat better

Vanilla-Fudge
I love this product

I love this product, it is good for my guts

30 Chewable Tablets
Velobiotic probiokid supplimens are the bestever

Velobiotic probiokid supplimens are the bestever, since we started till now i have seen a huge difference on my son’s daily food routine,he eat properly and he is active through out the day even atschool he has improved meaning his focus has also improved

30 Capsules
Love the real science behind these probiotics

Love the fact that there is real science behind the development of the probiotics and I can be confident that the product is going to work!
I use these every day for my gut and can feel the difference.

The

The best!

Awesome healing

I had bloating and constipation. Drinking these capsules within day 3 I saw a huge difference. No more bloating, I go to the toilet 2 times in a day now. I'm so happy, I purchased this product. The other probiotic brands did not work for me. I'm sticking to velobiotics forever. I can't wait to buy the 60 capsules. I'm truly grateful for this product to more healing of my gut. 🥂

Track your order